Part:BBa_K2719003:Experience

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Applications of BBa_K2719003

This part was cloned on pSB1C3 by restriction with EcoRI and PstI and transformed into E.coli DH5a, obtaining white colonies (see figure 1).

Figure 1. Colonies transformed with optimized GST.

Latter it has extracted and run on an agarose gel electrophoresis (see figure 2).

Figure 2. 0.85% agarose gel with GelRed, MW)NEB 1Kb DNA Ladder, 1)Extraction GST Optimized (BBa_K2719003), 2)Extraction GST Optimized (BBa_K2719003) duplicate.

Finally it was treated with NotI and run on an agarose gel electrophoresis to prove the presence of insert (see figure 3).

Figure 3. 0.85% agarose gel with GelRed, MW)NEB 1Kb DNA Ladder, 1)Restriction GST Optimized with NotI (BBa_K2719003), 2)Restriction GST Optimized with Not I (BBa_K2719003) duplicate.

User Reviews

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